Practice Subculturing (passaging) (3.3.1) - Chapter 3: Cell Culture Technology

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9 questions

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6 sample questions

Question 1.
What is subculturing?
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Think about why we need to do this in cell cultures.
Question 2.
What technique is commonly used to detach adherent cells?
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What enzyme might come to mind?
3Question 3.
What is the main purpose of subculturing cells?
- To increase cell density
- To maintain healthy growth
- To produce more waste
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Consider the needs of cells in culture.
4Question 4.
True or False: Primary cells can be subcultured indefinitely.
- True
- False
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Think about the life span of the cells.
Question 5.
You are tasked with maintaining a cell line that shows signs of overconfluence. Describe the appropriate steps you would take to subculture these cells and ensure their optimal health.
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Focus on each step and the importance of sterility.
Question 6.
During a routine subculture, you accidentally introduced a potential contaminant. What actions should you take to address the contamination?
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Think about contamination sources and how to mitigate them.

5 more questions in this section. Enroll free to practise them all.