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2 cards from this section. Quick, good the night before a test.
Define absorbance in the context of spectrophotometry.
Think about light interaction with the solution.
What is the formula of the Beer-Lambert Law?
What relationship does absorbance represent?
What does the Beer-Lambert Law state?
Remember the key variables in the Beer-Lambert Law.
True or False: Molar absorptivity is the same for all substances.
Think about how different substances absorb light.
A researcher prepares a calibration curve for a dye solution, yielding absorbance values of 0.1, 0.2, and 0.3 for concentrations of 0.01, 0.02, and 0.03 mol dm⁻³ respectively. If an unknown sample has an absorbance of 0.25, what is its concentration?
How can you use the known concentrations to determine the unknown?
During an experiment, deviations from the Beer-Lambert Law were noted at a concentration above 0.05 mol dm⁻³. What could account for this anomaly?
Consider what happens to light passage in concentrated solutions.
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