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3 cards from this section. Quick, good the night before a test.
What is the absorption equation used to determine concentration?
Identify the variables involved.
If the slope (m) is 2.0 and intercept (b) is 0, what is the concentration for an absorbance of 0.6?
Rearrange the absorption equation.
What does A stand for in the equation A = mC + b?
Think about the basic measurements in spectrometry.
True or False: The slope of the calibration curve decreases as concentration increases.
Consider how absorbance relates to concentration.
A sample measured absorbance of 0.750 with a slope of 1.500 ± 0.020 and an intercept of 0.050 ± 0.005. Determine the concentration and its associated uncertainty, providing all calculations done.
Use derivative methods for propagating uncertainties.
If you had a linear calibration curve but when plotting values you found deviations at high concentrations, what methods could you use to correct this and ensure reliability?
Think about how to adjust your measurements to fit within regard of Beer’s law.
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