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2 cards from this section. Quick, good the night before a test.
What does UV-Visible Spectrophotometry measure?
Think about the interaction between light and substances.
What is the Beer-Lambert Law?
It connects absorbance (A), molar absorptivity (ε), concentration (c), and path length (ℓ).
What does UV-Visible Spectrophotometry primarily measure?
Consider what the primary focus of the instrument is.
True or False: The Beer-Lambert Law states that absorbance is independent of concentration.
Think about how absorbance is affected by how much of a substance is present.
You have a dye with a molar absorptivity of 200 L/(mol·cm) and a cuvette path length of 1 cm. If the absorbance of the solution is 0.8, calculate the concentration of the dye.
Make sure to rearrange the Beer-Lambert Law formula.
An analytical chemist prepares a calibration curve and finds a linear relationship between absorbance and concentration. Discuss how they would determine if their findings are valid and reliable.
Think about the importance of repeatability in experiments.
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