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2 cards from this section. Quick, good the night before a test.
Define λ_max.
Think about what wavelength would give the strongest signal for a compound.
What is the purpose of a calibration curve?
This curve connects measured values to concentrations.
What does λ_max indicate?
Think about which point on a graph would give you the highest value.
True or False: The calibration curve represents a direct linear relationship between absorbance and concentration.
Consider the graphical representation of the data.
A standard calibration curve for a color solution has points (1 mg/L, 0.1 A) and (5 mg/L, 0.5 A). Calculate the slope and intercept of the best-fit line. Show your work.
Draw the graph to visualize the relationships, and apply the slope-intercept formula.
An unknown sample shows an absorbance of 0.45 A at λ_max. If your calibration curve shows the equation A = 0.1C (where A is absorbance and C is concentration in mg/L), calculate the concentration of the unknown sample and propagate uncertainties.
Look at both the slope and the absorbance value for calculations.
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