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4 cards from this section. Quick, good the night before a test.
What is the purpose of a calibration curve in quantitative fluorescence?
Think about how we measure concentration from intensity.
Define the inner filter effect.
What happens at high concentrations?
What is a primary use of a calibration curve in fluorescence spectroscopy?
Think about how calibration aligns with measuring concentration.
True or False: The inner filter effect only occurs at low concentrations of analyte.
Consider what happens to light absorption as concentration increases.
A solution exhibits an absorbance of 0.15 at excitation and 0.10 at emission wavelengths. Calculate the corrected fluorescence intensity assuming a measured intensity of 0.70.
Remember to average the absorbances.
If the Stern–Volmer constant K_SV is found to be 1.2, with a quencher concentration of 0.05 M, calculate the ratio of intensities I₀/I.
Revisit how to apply the Stern–Volmer equation.
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