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2 cards from this section. Quick, good the night before a test.
What is Beer’s law?
Think about how absorbance relates to concentration.
What does the path length (L) refer to?
Consider what happens to light as it passes through the solution.
What does Beer’s law relate to?
Consider what aspect of light measurement is being defined.
True or False: A calibration curve can be used to find unknown concentrations.
Think about why we create a graph.
You are given a series of absorbance data for a solution: 0.12, 0.25, 0.45, 0.60. If these correspond to concentrations of 0.5, 1.0, 1.5, and 2.0 M, determine the slope of the calibration curve and predict the concentration for an unknown sample with an absorbance of 0.38.
Identify how changes in absorbance relate to changes in concentration.
An unknown solution gives an absorbance of 0.87 and has associated uncertainties of ±0.02. If the calibration curve slope is 0.45 with an uncertainty of ±0.01, calculate the concentration of the unknown and the propagated uncertainty.
Follow the steps for uncertainty propagation diligently.
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