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2 cards from this section. Quick, good the night before a test.
What is a calibration curve?
Think about how light absorption relates to concentration.
What does molar absorptivity (ε) represent?
Consider the unit L mol⁻¹ cm⁻¹.
What does a calibration curve plot?
Consider the relationship between light absorption and concentration.
True or False: Molar absorptivity is constant for all concentrations.
Think about how absorption might change at higher concentrations.
If you prepared a calibration curve with the following concentrations and measured absorbances: 1.00 mg/L (0.102), 2.00 mg/L (0.204), 6.00 mg/L (0.610), and 8.00 mg/L (0.812). Using a linear regression fit, what is the derived equation, and how would you calculate the concentration of an unknown with an absorbance of 0.550?
Use the slope from your calculations to find the unknown concentration.
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