Practice What Are They? (1.1) - Tools and Techniques in Genetic Engineering

Practice: What Are They?

Lesson 1.1Tools and Techniques in Genetic Engineering

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10 questions

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6 sample questions

Question 1.
What are restriction enzymes?
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Think about their cutting function.
Question 2.
Give an example of a restriction enzyme.
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What enzyme cuts the GAATTC sequence?
3Question 3.
What do restriction enzymes specifically recognize?
- Protein sequences
- Lipids
- DNA sequences
- RNA sequences
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Focus on the type of molecule they act upon.
4Question 4.
True or False: Restriction enzymes are only found in human cells.
- True
- False
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Remember their purpose in bacteria.
Question 5.
Given a DNA sequence and a restriction enzyme, predict the fragments that will result after the cut. For example, how would EcoRI cut the sequence GAATTC?
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Draw out the sequence to visualize the cut.
Question 6.
Design an experiment using restriction enzymes to clone a gene from one organism into a plasmid vector. Outline the steps and mention how you would confirm successful cloning.
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Think about each of the key steps in molecular cloning and the tools you would use.

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