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4 cards from this section. Quick, good the night before a test.
What is the role of restriction enzymes in genetic engineering?
Think about their function in DNA manipulation.
What does DNA ligase do?
Consider what happens after DNA is cut.
What do restriction enzymes do?
Recall the scissors analogy we discussed.
True or False: DNA ligase is responsible for cutting DNA.
Think about the functions of ligase versus restriction enzymes.
You have a DNA segment that you need to clone into a plasmid. Describe the steps you will take using restriction enzymes and ligase. Include how you would visualize the success of your cloning attempt.
Think about the enzymes’ functions and the process of DNA manipulation.
Imagine you are developing a new PCR protocol. Discuss how you would ensure specificity and efficiency during the PCR process.
Recall the PCR steps and their thermal conditions.
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