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4 cards from this section. Quick, good the night before a test.
What do restriction enzymes do?
Think about the enzymes that 'restrict' or limit DNA.
What does DNA ligase do?
Remember: cutting and pasting!
What is the function of restriction enzymes?
Think about what 'restriction' does to a sequence.
True or False: DNA ligase can only join DNA fragments from the same organism.
Consider what recombinant DNA means.
If you have a DNA sequence cut by a restriction enzyme resulting in fragments 500bp, 200bp, and 300bp, how would gel electrophoresis visualize these fragments? Predict the order based on size.
Remember, smaller pieces move faster in the gel!
Given that restriction enzymes can create sticky ends or blunt ends, discuss the advantages of using sticky ends in DNA ligation. Why are they often preferred?
Think about how puzzle pieces fit together—sticky ends are like pieces that have edges fitting into each other.
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