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3 cards from this section. Quick, good the night before a test.
Define restriction enzymes in your own words.
What do they do to DNA?
Name one example of a restriction enzyme.
Think of a commonly used enzyme.
What do restriction enzymes do?
Think about their core function in genetic engineering.
True or False: Restriction enzymes are found in plants.
Where do you think they were first discovered?
Describe how you would use a restriction enzyme to clone a gene of interest from one organism into a plasmid for bacterial expression.
What steps are involved in gene cloning?
You have a DNA sequence that you suspect is recognized by a restriction enzyme. Outline the process of determining if it can be cut by that enzyme.
What tools could assist in matching sequences?
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