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4 cards from this section. Quick, good the night before a test.
What do restriction enzymes do?
Think about tools used in a laboratory.
What is the function of DNA ligase?
Consider what happens after DNA is cut.
What is the main function of restriction enzymes?
Think about how we manipulate DNA.
True or False: DNA ligase is used to cut DNA strands.
Remember the role of ligase compared to restriction enzymes.
Design an experiment where you would use restriction enzymes and ligases to create a recombinant plasmid. Describe the steps you would take.
Consider the role of each tool in the experimental setup.
Explain how gel electrophoresis can be used to verify the success of a PCR amplification. What would you look for in the results?
Remember how DNA size correlates with distance traveled in the gel.
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