Practice Chapter Summary (6) - Tools and Techniques in Genetic Engineering

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6 sample questions

Question 1.
What do restriction enzymes do?
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Think about how these enzymes are like scissors for DNA.
Question 2.
What is the role of DNA ligase?
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Consider its function when DNA has been cut.
3Question 3.
What enzyme cuts DNA?
- DNA Ligase
- Restriction Enzyme
- Taq Polymerase
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Think about what is used in genetic engineering to manipulate DNA.
4Question 4.
True or False: PCR only amplifies RNA.
- True
- False
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Consider what PCR stands for.
Question 5.
You have a DNA sample that consists of different fragments. How would you determine the sizes of these fragments?
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Remember how smaller fragments move faster in the gel.
Question 6.
You want to insert a gene into a plasmid for cloning. Explain the steps using restriction enzymes and ligase.
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Think about the importance of matching the cut ends.

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