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4 cards from this section. Quick, good the night before a test.
What do restriction enzymes do?
Think about how scissors cut paper but for DNA.
What does DNA ligase do?
Think of how glue bonds pieces of paper.
What is the main function of restriction enzymes?
Think about what they do, like scissors for DNA.
True or False: DNA ligase is used to cut DNA strands.
Remember that ligase pastes together, while restriction enzymes cut.
Design a simple experiment using restriction enzymes and DNA ligase to create a recombinant plasmid. Outline the steps you would take.
Remember the roles of each enzyme in cutting and pasting DNA.
In a forensic lab, how would you utilize PCR and gel electrophoresis to analyze a DNA sample from a crime scene?
Think about amplifying small quantities of DNA into larger, analyzable amounts.
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