Practice Application (1.3) - Tools and Techniques in Genetic Engineering

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11 questions

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6 sample questions

Question 1.
What do restriction enzymes do?
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Think about how bacteria defend themselves.
Question 2.
What is the role of DNA ligase?
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Consider what is needed to 'paste' DNA.
3Question 3.
What is the primary function of restriction enzymes?
- Cut DNA
- Join DNA
- Amplify DNA
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Think about what they do in bacteria.
4Question 4.
PCR is used to _____ DNA.
- Cut
- Amplify
- Separate
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What happens to DNA amounts during PCR?
Question 5.
Imagine you're tasked with cloning a gene. Describe the sequence of actions you'd take using restriction enzymes, DNA ligase, and PCR to create recombinant DNA.
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Think of the tools as a step-by-step process where each has a crucial role.
Question 6.
Design an experiment to analyze genetic material obtained from a crime scene using PCR and gel electrophoresis. Outline your method and expected results.
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Focus on the flow: collection, amplification, and analysis.

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