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2 cards from this section. Quick, good the night before a test.
What is a vector in recombinant DNA technology?
Think of it as a delivery vehicle.
What enzyme is responsible for joining DNA fragments?
It has 'ligate' in its name.
What is the primary role of a vector in recombinant DNA technology?
Think about what happens to the gene after it is inserted.
True or False: DNA ligase is necessary for the successful insertion of a gene into a vector.
Remember its function in the joining process.
Design an experiment that uses a vector to insert a gene of interest into a bacterial cell. What steps would you include, and why is each step critical?
Consider the overall goal of gene cloning.
Evaluate the impact of not using DNA ligase in the vector insertion process. What are the consequences?
Think about the importance of stability in recombinant DNA.
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