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4 cards from this section. Quick, good the night before a test.
What does 2D Gel Electrophoresis separate proteins by?
Think about the two dimensions mentioned.
What is the primary purpose of Western Blotting?
What technique do we use mainly for verification?
What does Mass Spectrometry primarily measure?
Think about what defines this method.
True or False: 2D Gel Electrophoresis can identify proteins by their amino acid sequences.
What does 2D Gel focus on?
Design an experiment using 2D Gel Electrophoresis and Mass Spectrometry to study protein changes in cancer cells. Describe the steps you'd take and the information you hope to gather.
Consider both the preparation and analysis phases.
Discuss how protein-protein interactions can influence cellular responses in a metabolic pathway. Provide examples of diseases that may be impacted by disrupted interactions.
Focus on connection and examples of dysfunctional interactions.
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