Practice Crispr-dcas9 Fusion Proteins (5.5.1) - Epigenetic Engineering and Regulation of Gene Expression

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6 sample questions

Question 1.
What does CRISPR stand for?
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Think about the function of these sequences in bacteria.
Question 2.
What is the role of dCas9?
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Consider what 'dead' in dCas9 implies.
3Question 3.
What is the primary function of dCas9?
- To cut DNA
- To methylate DNA
- To bind DNA without cutting
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Remember what 'dead' indicates in dCas9.
4Question 4.
True or False: dCas9-TET1 leads to gene silencing.
- True
- False
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Think about the process of methylation.
Question 5.
Propose a strategy to use dCas9-TET1 in therapy for a gene associated with a neurological disorder. What steps would you take?
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Think about how the modification would impact gene expression.
Question 6.
Discuss the potential risks of using CRISPR-dCas9 in germline editing. What safeguards would you implement?
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Consider both scientific and ethical dimensions of germline editing.

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