Practice Guide Rna (grna) Design And Pam Sequences (3) - CRISPR-Cas Systems and Genome Editing Tools

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6 sample questions

Question 1.
What does gRNA stand for?
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Consider what it's guiding.
Question 2.
What does PAM stand for?
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Think about the role of PAM in CRISPR.
3Question 3.
What is the length of a typical gRNA sequence?
- 15 nt
- 20 nt
- 25 nt
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Think about how specific you need to be in a targeting sequence.
4Question 4.
Is PAM necessary for the functioning of Cas enzymes?
- True
- False
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Remember the role of locks and keys in binding.
Question 5.
Given a target DNA sequence of 'ACGTTAGC', suggest a potential gRNA sequence and explain its importance.
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Ensure to analyze the area next to the target DNA for PAM correctness.
Question 6.
Critically analyze how changes in either gRNA or PAM sequence could affect the CRISPR editing outcome.
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Consider the Keystones of CRISPR: gRNA leads, PAM supports.

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