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4 cards from this section. Quick, good the night before a test.
What is the primary function of PCR?
Think of PCR as a DNA copier.
What does RT-PCR stand for?
It involves RNA.
What is the primary purpose of PCR?
Consider what PCR literally does.
True or False: RT-PCR is used exclusively for DNA amplification.
Focus on the 'RT' part.
Design a PCR experiment to amplify a gene of interest from a genomic DNA template. Outline the steps and rationale for each.
Think about each PCR step.
Discuss how you would apply qPCR in a project to test for viral load in patient samples.
Consider starting with RNA and the need for quantification.
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