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4 cards from this section. Quick, good the night before a test.
What are restriction enzymes used for?
What is their primary function?
What do sticky ends refer to?
Think about the shape of the cut.
What do restriction enzymes do?
Think about the action these enzymes perform on DNA.
True or False: Sticky ends are easier to join than blunt ends.
Consider the structural differences in the ends.
You need to clone a gene with a sticky end using EcoRI into a plasmid that has also been cut with EcoRI. Describe the steps you would take.
Consider what other components might be required in the process.
Explain how you would resolve a situation where you accidentally used a blunt-end producing restriction enzyme instead of a sticky-end one. How might this affect the cloning efficiency?
Think about how docking works with different cut types.
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