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4 cards from this section. Quick, good the night before a test.
What does PCR stand for?
Think of what PCR does!
What is the purpose of restriction enzymes?
Consider how DNA is prepared in experiments.
What technique is used for amplifying DNA?
Think about the name of the technique.
True or False: Restriction enzymes can create sticky ends.
Consider how the cuts are made and how DNA would fit together.
How would you design an experiment to measure the expression of a specific gene under different environmental conditions using qPCR?
Think about the controls you'll need in your setup.
Discuss the implications of using a recombinant plasmid versus a BAC for inserting a very large DNA sequence.
Consider what each vector type can handle.
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