Practice Dna Ligase (3.2) - Advanced Molecular Biology Techniques in Genetic Engineering

Practice: DNA Ligase

Lesson 3.2Advanced Molecular Biology Techniques in Genetic Engineering

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9 questions

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3 cards from this section. Quick, good the night before a test.
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6 questions · about 4 min · pass mark 70%. Hints available.
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Quiz
3 questions · about 2 min · pass mark 70%. Hints available.
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6 sample questions

Question 1.
What is the role of DNA ligase?
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Think about what happens in DNA replication.
Question 2.
What type of ends do sticky ends have?
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How do they differ from blunt ends?
3Question 3.
What is the main function of DNA ligase?
- To synthesize RNA
- To join DNA fragments
- To cut DNA
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Consider what ligase means!
4Question 4.
True or False: Sticky ends are more efficient in ligation than blunt ends.
- True
- False
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Think about their structure.
Question 5.
Given a scenario where you have plasmids with sticky ends from EcoRI and blunt ends from another enzyme, explain how you would approach cloning a gene into a plasmid and why one method is preferred over the other.
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Consider the nature of ligation processes.
Question 6.
In a DNA ligation experiment, it's found that the ligation efficiency is very low. What factors could contribute to this, and how would you troubleshoot the problem?
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Review key components involved in the ligation process.

5 more questions in this section. Enroll free to practise them all.