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5 cards from this section. Quick, good the night before a test.
What does PCR stand for?
Think of what process this is involved in regarding DNA.
What do restriction enzymes do?
Consider what parts of the DNA are modified during cloning.
What is the primary function of PCR?
Think about what this technique is best known for.
True or False: Restriction enzymes can cleave DNA at random locations.
Recall how these enzymes are precise in their action.
You have a gene that you want to amplify for cloning. Describe the steps you would take using PCR and how you would analyze the results.
Consider starting with template DNA and the choice of primers.
An experiment requires you to create a knock-out mouse. Explain the process using recombinase technology.
Focus on how the recombinase targets specific DNA sequences.
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