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4 cards from this section. Quick, good the night before a test.
What is a plasmid?
Think of it as a mini ring of DNA.
What does BAC stand for?
It has to do with bacteria!
What is the primary function of a cloning vector?
Think about what they do in genetic engineering!
True or False: BACs can only carry small DNA inserts.
Consider what 'large' means in the context of DNA.
Design an experiment to clone a gene of interest using a plasmid vector. What would be your steps and considerations?
Think about how you would ensure your gene is successfully expressed in the host cells.
Evaluate the pros and cons of using a viral vector for a gene therapy project. What safety considerations must be addressed?
Consider both the benefits and the potential risks that come with using viral systems.
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