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3 cards from this section. Quick, good the night before a test.
Define UV absorbance in your own words.
Think about what absorption means.
What is the formula for calculating absorbance?
Recall the terms I0 and I.
What does UV absorbance measure?
Think about the purpose of measuring light in spectroscopy.
True or False: Higher absorbance corresponds to lower concentration.
Consider how concentration affects absorbance.
A solution shows an absorbance of 1.2 at a wavelength of 260 nm. If a known standard solution has an absorbance of 0.2 at the same wavelength for a concentration of 0.05 M, calculate the concentration of the unknown solution.
Utilize the direct proportion between absorbance and concentration.
If a sample absorbs UV light at 280 nm strongly, how would its absorbance change if diluted by half?
Recall what happens to absorbance as concentration changes following linear relationships.
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