Practice Indian Institute Of Technology-madras (1.3) - Analysis Methods – Liquid Chromatography

Practice: Indian Institute of Technology-Madras

Lesson 1.3Analysis Methods – Liquid Chromatography

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10 questions

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6 sample questions

Question 1.
What is the main advantage of liquid chromatography over gas chromatography?
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Think about the states of matter for the samples.
Question 2.
Name a common detector used in liquid chromatography.
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Consider the types of light interactions.
3Question 3.
What is the main function of the mobile phase in liquid chromatography?
- To change the column temperature
- To transport the analytes
- To ignite the samples
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What carries the samples during the chromatography process?
4Question 4.
True or False: Liquid chromatography does not require the vaporization of samples.
- True
- False
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What is a major difference between liquid and gas chromatography?
Question 5.
Design a liquid chromatography experiment to analyze a complex mixture of hormones in a biological sample. Specify your choice of mobile phase and detection method, explaining your reasoning.
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Consider the properties of the hormones and their expected concentration.
Question 6.
Explain how you would troubleshoot a liquid chromatography setup that shows inconsistent peak shapes in the chromatogram.
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What mechanical and chemical factors can affect chromatography results?

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