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3 cards from this section. Quick, good the night before a test.
What is the partition constant (K)?
Think about how compounds interact with different phases.
What happens to retention time if the partition constant increases?
Consider the concept of attraction between phases.
What does a high partition constant (K) indicate?
Think about which phase analytes prefer.
True or False: Adjusting the temperature in chromatography can change the partition constant.
Consider how heat affects compounds in solution.
You have a complex mixture of volatile and non-volatile compounds. Describe how you would set up a chromatography experiment to effectively analyze both types, including any steps you would take to dynamically control the process.
Think about the characteristics that each type of compound has in relation to their phase preferences.
The results from your chromatography analysis show overlap in peak retention times for two different analytes. Discuss possible strategies to resolve this issue without reanalyzing the samples.
Identify how changing each parameter can influence separation of analytes!
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