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2 cards from this section. Quick, good the night before a test.
What are the two phases involved in chromatography?
Think about what stays fixed and what moves.
What is the partition constant (K)?
K affects retention time!
What is the role of the mobile phase in chromatography?
Identify which phase moves through the system.
True or False: Calibration helps in identifying compounds based solely on their retention time.
Recall the limitations of relying on retention time.
You are given a chromatogram with overlapping peaks. Describe how you would identify the individual components present in the mixture.
How can you separate overlapping components?
Explain how you would adjust the conditions of your gas chromatography setup to better separate two compounds that have very similar retention times.
Think about ways to manipulate K and temperatures.
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