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3 cards from this section. Quick, good the night before a test.
What is chromatography?
Think about why we need to separate compounds.
Name the two phases involved in chromatography.
One phase is fixed, the other moves.
What is the main purpose of chromatography?
Think about the purpose of this analysis.
True or False: The stationary phase is the phase that moves in chromatography.
Remember the definitions of each phase.
Given a mixture of three compounds with different partition coefficients (K1=1.5, K2=2.0, K3=0.5), predict the order they will elute from a column.
Consider each compound's affinity for the stationary phase.
Develop a troubleshooting guide for chromatography analysis resulting in poor separation.
Think about all steps in the chromatography process to identify potential issues.
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