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5 cards from this section. Quick, good the night before a test.
What is the function of restriction enzymes?
Think about how scissors work with paper.
What is a cloning vector?
Consider a vehicle for DNA.
What is the main function of restriction enzymes?
Think about their comparison to scissors.
True or False: Competent cells can naturally uptake DNA without any treatments.
Consider how natural processes differ from modified ones.
How would you design an experiment to test the efficiency of different cloning vectors in transferring a gene of interest into a bacterial host?
Think of measurable criteria like colony counts or growth rates.
Explain the molecular mechanisms that enable a host cell to maintain a new piece of DNA after transformation.
Consider the importance of replication origins and chromosomal integration.
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