Practice Processes Of Recombinant Dna Technology (9.3) - Biotechnology : Principles and Processes

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6 sample questions

Question 1.
What is the first step in recombinant DNA technology?
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Think about what needs to be done with DNA before cutting it.
Question 2.
Name one enzyme used in the cutting of DNA.
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It's a type of enzyme that can cut DNA.
3Question 3.
What is the purpose of restriction enzymes in recombinant DNA technology?
- To amplify DNA
- To cut DNA
- To purify proteins
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Think about what happens before ligation.
4Question 4.
True or False: PCR can produce billions of copies of DNA in a short amount of time.
- True
- False
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Remember the exponential nature of PCR.
Question 5.
Given the following DNA fragment: ATCGGCAATCGG, what would the complementary strand be, and how would it be useful in the context of recombinant DNA technology?
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Think about base pairing rules in DNA.
Question 6.
Design an experiment to evaluate the efficiency of a new restriction enzyme. What steps would you include from start to finish?
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Consider all the steps we discussed in processing recombinant DNA.

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