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2 cards from this section. Quick, good the night before a test.
What is the purpose of isolating DNA?
Think about what DNA holds that is essential in biology.
Name one enzyme used to lyse bacterial cells.
This enzyme breaks down the cell wall of bacteria.
What is the first step in isolating DNA from a cell?
Think about the necessary process needed to access the DNA.
True or False: Ribonuclease is used to break down proteins during DNA isolation.
Recall which macromolecule each enzyme acts upon.
Design a step-by-step protocol to isolate DNA from a given organism while considering potential contaminants at each stage.
Consider the type of organism and the required enzymes.
Explain how impurities can affect an experiment involving PCR. What protocols would you implement to ensure high purity of the extracted DNA?
What specific tests could be done post-isolation to evaluate purity?
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