Practice Cutting Of Dna At Specific Locations (9.3.2) - Biotechnology : Principles and Processes

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6 sample questions

Question 1.
What is a restriction enzyme?
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Think about what they do to DNA.
Question 2.
Give an example of a restriction enzyme.
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Remember the acronym for Escherichia coli.
3Question 3.
What do restriction enzymes do?
- Cut DNA at specific sequences
- Join DNA fragments
- Visualize DNA
- Amplify DNA
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Recall the definition of restriction enzymes.
4Question 4.
True or False: Sticky ends can help join different DNA fragments.
- True
- False
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Consider how sticky ends are formed.
Question 5.
Explain how you would design an experiment to evaluate the effectiveness of a specific restriction enzyme on a given DNA sample.
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Consider what steps are involved in preparing and analyzing DNA.
Question 6.
Discuss the implications of using a restriction enzyme that does not recognize a specific sequence. What effects would it have on cloning efforts?
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What happens if you can't cut the DNA where needed?

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