Practice Cloning Vectors (9.2.2) - Biotechnology : Principles and Processes

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6 sample questions

Question 1.
What is a cloning vector?
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Think about how biotechnologists can manipulate genes.
Question 2.
Name one feature a cloning vector must have.
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Where does the replication of DNA begin?
3Question 3.
What is a primary feature of cloning vectors?
- They contain genes for synthesizing proteins
- They cannot replicate independently
- They have an origin of replication
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Remember what helps the vector reproduce inside the cell.
4Question 4.
True or False: Selectable markers are used to identify cells that did not take up the cloning vector.
- True
- False
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Think about how scientists can tell which cells are transformed.
Question 5.
Design a new cloning vector for a gene of interest that requires high expression in a specific bacterial strain. What features would you include?
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Consider what will help the bacteria efficiently express the gene.
Question 6.
Explain the implications of choosing the wrong selectable marker for a cloning vector.
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Think about the role of selection in genetic engineering.

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